Doxorubicin-induced F-actin reorganization in cofilin-1 (nonmuscle) down-regulated CHO AA8 cells.

The actin cytoskeleton plays an important role in many cellular processes, including cell mortality, mitosis, cytokinesis, intracellular transport, endocytosis and secretion but also is involved in gene transcription. The dynamics of the actin cytoskeleton is controlled by different classes of actin-binding proteins (ABPs) which regulate the polymerization of actin filaments. In this report we used siRNA against cofilin-1 (nonmuscle) to demonstrate the effect of cofilin on the nuclear and cytoplasmic actin pools in CHO AA8 cells after exposition to various concentrations of doxorubicin. The immunofluorescence studies showed doxorubicin dose dependent tendency to formation the multinucleated giant cells, but also the increase of fluorescence intensity of cofilin in nuclei of untransfected cells. Induction of cell death with doxorubicin treatment in untransfected cells revealed both mitotic catastrophe (in both lower and higher doxorubicin doses) and apoptosis (mostly in higher doxorubicin doses), whereas among cofilin-1 down-regulated cells we observed only mitotic catastrophe. The results suggest that cofilin has apoptosis-inducing ability and that mitotic catastrophe is independent from F-actin content in cell nucleus. In this point of view we conclude that different mechanisms of chromatin reorganization are involved in these two processes. Moreover, we suppose that apoptosis and mitotic catastrophe are independent from each other

Electrocardiography and echocardiography in athletic heart imagining

The aim of the study was evaluation of electrocardiographic and echocardiographic parameters in athletes. „Athletic heart” characteristics were compared with fit persons’ heart. 96 athletes participated in the study. Sportsmen were divided into: static (S), dynamic (D) and composite (SD) exercise groups and Polish (I), European (II), World (III) champions. 30 students from Sport Academy formed the control group (K). Electrocardiographic and echocardiographic examination were performed in everyone. As regards the type of exercise, the end-systolic left ventricular (LV) dimension was smaller in S in comparison with D and SD (28.9 vs 32.2 and 32.68 mm; P<0.05). LV mass was bigger in D in comparison with K (273.2 vs 218.6 g; P<0.05). Medium Pulmonary Artery Pressure (MPAP) in S and SD was lower in respect to D and K (11.75; 11.08 vs 15.52; 17.43 mm Hg; P<0.05). We observed lower heart rate in D, SD in comparison with K (58.64; 60.54 vs 68.8; P<0.05), bigger R wave amplitude in V5 (RV5) (21.65; 23.5 vs 15.03 mm; P<0.05) and V6 (RV6) (23.5 vs 15.3 mm; P<0.05) in group S in respect to K. LV mass was bigger in III than in K (261.3 vs 218.6 g; P<0.05). MPAP was lower in I and II in comparison with K (11.42; 13.13 vs 17.43 mmHg; P<0.05). HR was lower in categories I, II than in K (61.32; 60.13 vs 68.8; P<0.05), RV5 was bigger in I in comparison with K (19.5 vs 15.03 mm; P<0.05). The electrocardiography and echocardiography proves to find some significant differences between athletic and fit persons’ heart especially as concerns MPAP, RV5, RV6 values

Zastosowanie manualnych technik osteopatycznych w leczeniu bólu pleców u kobiet w ciąży

Changes in body posture, musculoskeletal disorders and somatic dysfunctions are frequently observed during pregnancy, especially ligament, joint and myofascial impairment. The aim of the paper is to present the use of osteopathic manipulative treatment (OMT) for back and pelvic pain in pregnancy on the basis of a review of the available literature. MEDLINE and Cochrane Library were searched in January 2014 for relevant reports, randomized controlled trials, clinical and case studies of OMT use in pregnant women. Each eligible source was verified and analyzed by two independent reviewers. OMT procedures appear to be effective and safe for pelvic and spinal pain management in the lumbosacral area in pregnant women.W trakcie ciąży u kobiet zachodzą zmiany w postawie ciała, układzie mięśniowo-szkieletowym oraz zaburzenia somatyczne. Najczęściej dochodzi do dysfunkcji więzadeł, stawów i powięzi. Celem pracy jest przedstawienie wykorzystania bezpiecznych osteopatycznych technik manualnych w leczeniu bólu pleców i miednicy u kobiet w ciąży, na podstawie przeglądu dostępnej literatury naukowej. Przegląd literatury przeprowadzono w bazach danych MEDLINE i Cochrane Library w styczniu 2014 roku. Do analizy wybrano prace poglądowe, randomizowane, kliniczne oraz studium przypadku dotyczące terapii manualnej u kobiet w ciąży. Każda praca była weryfikowana i analizowana przez dwóch niezależnych badaczy. Osteopatyczne techniki manualne (OMT) wydają się być skuteczną i bezpieczną terapią w bólach odcinka lędźwiowo-krzyżowego kręgosłupa i miednicy u kobiet w ciąży

Using muon rings for the optical throughput calibration of the SST-1M prototype for the Cherenkov Telescope Array

Imaging Atmospheric Cherenkov Telescopes (IACTs) are ground-based instruments devoted to the study of very high energy gamma-rays coming from space. The detection technique consists of observing images created by the Cherenkov light emitted when gamma rays, or more generally cosmic rays, propagate through the atmosphere. While in the case of protons or gamma-rays the images present a filled and more or less elongated shape, energetic muons penetrating the atmosphere are visualised as characteristic circular rings or arcs. A relatively simple analysis of the ring images allows the reconstruction of all the relevant parameters of the detected muons, such as the energy, the impact parameter, and the incoming direction, with the final aim to use them to calibrate the total optical throughput of the given IACT telescope. We present the results of preliminary studies on the use of images created by muons as optical throughput calibrators of the single mirror small size telescope prototype SST-1M proposed for the Cherenkov Telescope Array.Comment: In Proceedings of the 34th International Cosmic Ray Conference (ICRC2015), The Hague, The Netherlands. All CTA contributions at arXiv:1508.0589

DigiCam - Fully Digital Compact Read-out and Trigger Electronics for the SST-1M Telescope proposed for the Cherenkov Telescope Array

The SST-1M is one of three prototype small-sized telescope designs proposed for the Cherenkov Telescope Array, and is built by a consortium of Polish and Swiss institutions. The SST-1M will operate with DigiCam - an innovative, compact camera with fully digital read-out and trigger electronics. A high level of integration will be achieved by massively deploying state-of-the-art multi-gigabit transmission channels, beginning from the ADC flash converters, through the internal data and trigger signals transmission over backplanes and cables, to the camera's server link. Such an approach makes it possible to design the camera to fit the size and weight requirements of the SST-1M exactly, and provide low power consumption, high reliability and long lifetime. The structure of the digital electronics will be presented, along with main physical building blocks and the internal architecture of FPGA functional subsystems.Comment: In Proceedings of the 34th International Cosmic Ray Conference (ICRC2015), The Hague, The Netherlands. All CTA contributions at arXiv:1508.0589

Software design for the control system for Small-Size Telescopes with single-mirror of the Cherenkov Telescope Array

The Small-Size Telescope with single-mirror (SST-1M) is a 4 m Davies-Cotton telescope and is among the proposed telescope designs for the Cherenkov Telescope Array (CTA). It is conceived to provide the high-energy ($>$ few TeV) coverage. The SST-1M contains proven technology for the telescope structure and innovative electronics and photosensors for the camera. Its design is meant to be simple, low-budget and easy-to-build industrially. Each device subsystem of an SST-1M telescope is made visible to CTA through a dedicated industrial standard server. The software is being developed in collaboration with the CTA Medium-Size Telescopes to ensure compatibility and uniformity of the array control. Early operations of the SST-1M prototype will be performed with a subset of the CTA central array control system based on the Alma Common Software (ACS). The triggered event data are time stamped, formatted and finally transmitted to the CTA data acquisition. The software system developed to control the devices of an SST-1M telescope is described, as well as the interface between the telescope abstraction to the CTA central control and the data acquisition system.Comment: In Proceedings of the 34th International Cosmic Ray Conference (ICRC2015), The Hague, The Netherlands. All CTA contributions at arXiv:1508.0589

Prototype of the SST-1M Telescope Structure for the Cherenkov Telescope Array

A single-mirror small-size (SST-1M) Davies-Cotton telescope with a dish diameter of 4 m has been built by a consortium of Polish and Swiss institutions as a prototype for one of the proposed small-size telescopes for the southern observatory of the Cherenkov Telescope Array (CTA). The design represents a very simple, reliable, and cheap solution. The mechanical structure prototype with its drive system is now being tested at the Institute of Nuclear Physics PAS in Krakow. Here we present the design of the prototype and results of the performance tests of the structure and the drive and control system.Comment: In Proceedings of the 34th International Cosmic Ray Conference (ICRC2015), The Hague, The Netherlands. All CTA contributions at arXiv:1508.0589

Interspecific introgression of MHC genes in Triturus newts: evidence from multiple contact zones

The major histocompatibility complex (MHC) genes are central to the adaptive immune response in vertebrates. Selection generally maintains high MHC variation because the spectrum of recognized pathogens depends on MHC polymorphism. Novel alleles favoured by selection originate by interallelic recombination or de novo mutations but may also be acquired by introgression from related species. However, the extent and prevalence of MHC introgression remain an open question. In this study, we tested for MHC introgression in six hybrid zones formed by six Triturus newt species. We sequenced and genotyped the polymorphic second exons of the MHC class I and II genes and compared their interspecific similarity at various distances from the centre of the hybrid zone. We found evidence for introgression of both MHC classes in the majority of examined hybrid zones, with support for a more substantial class I introgression. Furthermore, the overall MHC allele sharing outside of hybrid zones was elevated between pairs of Triturus species with abutting ranges, regardless of the phylogenetic distance between them. No effect of past hybrid zone movement on MHC allele sharing was found. Finally, using previously published genome-wide data, we demonstrated that MHC introgression was more extensive than genome-wide introgression, supporting its adaptive potential. Our study thus provides evidence for the prevalence of MHC introgression across multiple Triturus hybrid zones, indicating that MHC introgression between divergent hybridizing species may be widespread and adaptive.Animal science

Транссиб: путешествие во времени

[For the English abstract and full text of the article please see the attached PDF-File (English version follows Russian version)].ABSTRACT The Organization for Cooperation between Railways (OSJD) in every possible way promotes the exchange of experience and study tours for representatives of participating countries and affiliated enterprises. Broad international contacts allow developing mutual understanding, strengthening business ties, transferring knowledge, maintaining historical continuity in the field of railway traffic. At the end of July last year a group of Polish railway workers returned from the Trans-Siberian Railway journey. Judging by the published notes, they had the opportunity to satisfy not only professional interest, but also got acquainted with the history of the Trans-Baikal Territory, the past of the Buryat and Mongolian people. The modern locomotive, unwillingly, became a «time machine» for travelers. Keywords: Trans-Siberian Railway, TransSib, Trans-Baikal, retrospective, OSJD.Текст аннотации на англ. языке и полный текст статьи на англ. языке находится в прилагаемом файле ПДФ (англ. версия следует после русской версии).Организация сотрудничества железных дорог (ОСЖД) всячески содействует популяризации обмена опытом и ознакомительных поездок для представителей стран-участниц и присоединённых предприятий. Широкие международные контакты позволяют развивать взаимопонимание, укреплять деловые связи, передавать знания, блюсти историческую преемственность в сфере железнодорожных сообщений. В конце июля минувшего года из поездки по Транссибу возвратилась группа польских железнодорожников. Судя по публикуемым заметкам, они имели возможность удовлетворить не только профессиональный интерес, но и познакомились с историей Забайкальского края, прошлым бурятского и монгольского народов. Современный локомотив поневоле стал для путешественников «машиной времени»